Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure.
Identifieur interne : 000478 ( Main/Exploration ); précédent : 000477; suivant : 000479Proteomic and metabolomic profiling of Valencia orange fruit after natural frost exposure.
Auteurs : Valeria E. Perotti [Argentine] ; Alejandra S. Moreno ; Karina E J. Trípodi ; Guillermo Meier ; Fernando Bello ; Mariángeles Cocco ; Daniel Vázquez ; Catalina Anderson ; Florencio E. PodestáSource :
- Physiologia plantarum [ 1399-3054 ] ; 2015.
English descriptors
- KwdEn :
- Alcohols (metabolism), Carbohydrate Metabolism, Carbohydrates, Carboxylic Acids (metabolism), Citrus sinensis (genetics), Citrus sinensis (metabolism), Flavonoids (metabolism), Freezing, Fruit (genetics), Fruit (metabolism), Gene Expression Regulation, Plant, Metabolomics, Plant Proteins (metabolism), Propanols (metabolism), Proteomics, RNA, Messenger (genetics), RNA, Plant (genetics).
- MESH :
- chemical , genetics : RNA, Messenger, RNA, Plant.
- chemical , metabolism : Alcohols, Carboxylic Acids, Flavonoids, Plant Proteins, Propanols.
- genetics : Citrus sinensis, Fruit.
- metabolism : Citrus sinensis, Fruit.
- Carbohydrate Metabolism, Carbohydrates, Freezing, Gene Expression Regulation, Plant, Metabolomics, Proteomics.
Abstract
The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.
DOI: 10.1111/ppl.12259
PubMed: 25132553
Affiliations:
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Le document en format XML
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Moreno</name></author><author><name sortKey="Tripodi, Karina E J" sort="Tripodi, Karina E J" uniqKey="Tripodi K" first="Karina E J" last="Trípodi">Karina E J. Trípodi</name></author><author><name sortKey="Meier, Guillermo" sort="Meier, Guillermo" uniqKey="Meier G" first="Guillermo" last="Meier">Guillermo Meier</name></author><author><name sortKey="Bello, Fernando" sort="Bello, Fernando" uniqKey="Bello F" first="Fernando" last="Bello">Fernando Bello</name></author><author><name sortKey="Cocco, Mariangeles" sort="Cocco, Mariangeles" uniqKey="Cocco M" first="Mariángeles" last="Cocco">Mariángeles Cocco</name></author><author><name sortKey="Vazquez, Daniel" sort="Vazquez, Daniel" uniqKey="Vazquez D" first="Daniel" last="Vázquez">Daniel Vázquez</name></author><author><name sortKey="Anderson, Catalina" sort="Anderson, Catalina" uniqKey="Anderson C" first="Catalina" last="Anderson">Catalina Anderson</name></author><author><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E" last="Podestá">Florencio E. 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Perotti</name><affiliation wicri:level="1"><nlm:affiliation>Centro de Estudios Fotosintéticos y Bioquímicos, CONICET, and Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, 2000, Argentina.</nlm:affiliation><country xml:lang="fr">Argentine</country><wicri:regionArea>Centro de Estudios Fotosintéticos y Bioquímicos, CONICET, and Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, 2000</wicri:regionArea><wicri:noRegion>2000</wicri:noRegion></affiliation></author><author><name sortKey="Moreno, Alejandra S" sort="Moreno, Alejandra S" uniqKey="Moreno A" first="Alejandra S" last="Moreno">Alejandra S. Moreno</name></author><author><name sortKey="Tripodi, Karina E J" sort="Tripodi, Karina E J" uniqKey="Tripodi K" first="Karina E J" last="Trípodi">Karina E J. Trípodi</name></author><author><name sortKey="Meier, Guillermo" sort="Meier, Guillermo" uniqKey="Meier G" first="Guillermo" last="Meier">Guillermo Meier</name></author><author><name sortKey="Bello, Fernando" sort="Bello, Fernando" uniqKey="Bello F" first="Fernando" last="Bello">Fernando Bello</name></author><author><name sortKey="Cocco, Mariangeles" sort="Cocco, Mariangeles" uniqKey="Cocco M" first="Mariángeles" last="Cocco">Mariángeles Cocco</name></author><author><name sortKey="Vazquez, Daniel" sort="Vazquez, Daniel" uniqKey="Vazquez D" first="Daniel" last="Vázquez">Daniel Vázquez</name></author><author><name sortKey="Anderson, Catalina" sort="Anderson, Catalina" uniqKey="Anderson C" first="Catalina" last="Anderson">Catalina Anderson</name></author><author><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E" last="Podestá">Florencio E. Podestá</name></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Alcohols (metabolism)</term><term>Carbohydrate Metabolism</term><term>Carbohydrates</term><term>Carboxylic Acids (metabolism)</term><term>Citrus sinensis (genetics)</term><term>Citrus sinensis (metabolism)</term><term>Flavonoids (metabolism)</term><term>Freezing</term><term>Fruit (genetics)</term><term>Fruit (metabolism)</term><term>Gene Expression Regulation, Plant</term><term>Metabolomics</term><term>Plant Proteins (metabolism)</term><term>Propanols (metabolism)</term><term>Proteomics</term><term>RNA, Messenger (genetics)</term><term>RNA, Plant (genetics)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Messenger</term><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Alcohols</term><term>Carboxylic Acids</term><term>Flavonoids</term><term>Plant Proteins</term><term>Propanols</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus sinensis</term><term>Fruit</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Carbohydrate Metabolism</term><term>Carbohydrates</term><term>Freezing</term><term>Gene Expression Regulation, Plant</term><term>Metabolomics</term><term>Proteomics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this study was to evaluate the response of orange fruit (Citrus sinensis var. Valencia Late) to freezing stress in planta, both immediately after the natural event and after a week, in order to understand the biochemical and molecular basis of the changes that later derive in internal and external damage symptoms. Using two-dimensional differential gel electrophoresis to analyze exposed and non-exposed fruit, 27 differential protein spots were detected in juice sacs and flavedo, among all comparisons made. Also, primary and secondary metabolites relative contents were analyzed in both tissues by gas chromatography-mass spectrometry and liquid chromatography-mass spectrometry, respectively. Proteins and compounds involved in regulatory functions, iron metabolism, oxidative damage and carbohydrate metabolism were the most affected. Interestingly, three glycolytic enzymes were induced by cold, and there was an increase in fermentation products (volatiles); all of that suggests that more energy generation might be required from glycolysis to counter the cold stress. Moreover, a notable increase in sugar levels was observed after frost, but it was not at the expense of organic acids utilization. Consequently, these results suggest a probable redistribution of photoassimilates in the frost-exposed plants, tending to restore the homeostasis altered by that severe type of stress. Isosinensetin was the most cold-sensitive secondary metabolite because it could not be detected at all after the frost, constituting a possible tool to early diagnose freezing damage.</div></front></TEI><affiliations><list><country><li>Argentine</li></country></list><tree><noCountry><name sortKey="Anderson, Catalina" sort="Anderson, Catalina" uniqKey="Anderson C" first="Catalina" last="Anderson">Catalina Anderson</name><name sortKey="Bello, Fernando" sort="Bello, Fernando" uniqKey="Bello F" first="Fernando" last="Bello">Fernando Bello</name><name sortKey="Cocco, Mariangeles" sort="Cocco, Mariangeles" uniqKey="Cocco M" first="Mariángeles" last="Cocco">Mariángeles Cocco</name><name sortKey="Meier, Guillermo" sort="Meier, Guillermo" uniqKey="Meier G" first="Guillermo" last="Meier">Guillermo Meier</name><name sortKey="Moreno, Alejandra S" sort="Moreno, Alejandra S" uniqKey="Moreno A" first="Alejandra S" last="Moreno">Alejandra S. Moreno</name><name sortKey="Podesta, Florencio E" sort="Podesta, Florencio E" uniqKey="Podesta F" first="Florencio E" last="Podestá">Florencio E. Podestá</name><name sortKey="Tripodi, Karina E J" sort="Tripodi, Karina E J" uniqKey="Tripodi K" first="Karina E J" last="Trípodi">Karina E J. Trípodi</name><name sortKey="Vazquez, Daniel" sort="Vazquez, Daniel" uniqKey="Vazquez D" first="Daniel" last="Vázquez">Daniel Vázquez</name></noCountry><country name="Argentine"><noRegion><name sortKey="Perotti, Valeria E" sort="Perotti, Valeria E" uniqKey="Perotti V" first="Valeria E" last="Perotti">Valeria E. Perotti</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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